High Throughput SNP Genotyping in Candidate Genes for Human Obesity
The aim of this subproject is to provide SNP genotyping for candidate genes identified by members of the obesity consortium. The high throughput genotyping laboratory at the GSF has been established as a central SNP genotyping facility in the NGFN and is based on mass spectrometry (Sequenom) which provides a cost efficient and flexible approach for SNP-based association studies in candidate genes. The working packages include interactive definition of SNP lists and assay design, the processing of samples for the high throughput process, SNP genotyping and data delivery in a standard format. SNP selection and haplotype tagging algorithms (htSNPs) will be provided for genes and regions where haplotype information is available through the HapMap project. If required, information on SNPs and haplotypes will be generated for candidate genes.
